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BIO 304 · Human Anatomy & Physiology

Fluid, Electrolyte & Acid-Base Balance

Urinary System · Module 15

A reference for the Fluid & Acid-Base video. Body fluid lives in compartments. Electrolytes are the ions that matter. Acid-base balance is a fast respiratory layer plus a slow renal layer working together.

How to use this sheet Toggle the toolbar above. Notes prints the full reference for review. Study prints as a fill-in-the-blank worksheet , print it, then write each definition while you watch the video or read your book. Quiz me is on-screen typing practice; type the term, click Reveal to check yourself.

Open spaced recall

By the end
  1. Distinguish intracellular fluid from extracellular fluid by volume and composition.
  2. Identify the major roles and regulators of sodium, potassium, calcium, and phosphate.
  3. Classify an acid-base disturbance as metabolic or respiratory and acidosis or alkalosis.
Anterior view of the body and face labeled with upper-body regions: cranial, frontal, orbital, nasal, buccal, oris, mental, cervical, acromial, deltoid, axillary, brachial, antecubital, antebrachial, carpal, digital, mammary, sternal, abdominal, umbilical.
Anterior · upper body & face
Anterior view of the body labeled with lower-body regions: pelvic, inguinal, pubic, coxal, pollex, femoral, patellar, fibular, crural, tarsal, plantar, digital toes, and hallux.
Anterior · lower body
Posterior view labeled occipital, cervical, scapular, vertebral, lumbar, sacral, glu#0B1530, femoral, popli#0B1530, sural, tarsal, calcaneal; lateral head view labeled otic, buccal, occipital, cervical.
Posterior & lateral head

Click any image to enlarge.


Fluid & Electrolytes

Body fluid (60% of body weight)

  • Intracellular fluid (ICF, ~40%)high K+, phosphate, Mg²⁺, protein
  • Extracellular fluid (ECF, ~20%)high Na+, Cl-, HCO₃-
  • Plasma (~5%)ECF inside blood vessels; has plasma proteins
  • Interstitial (~15%)ECF outside vessels; bathes cells

Sodium (Na+)

  • Normal serum135-145 mEq/L
  • Main extracellular cationsets ECF osmolarity
  • RegulatorsADH (water), aldosterone (Na+ reabsorption), ANP (Na+ excretion)
  • Hyponatremiacerebral edema; seizures if rapid
  • Hypernatremiacellular shrinkage; thirst

Potassium (K+)

  • Normal serum3.5-5.0 mEq/L
  • Main intracellular cationtiny serum changes have big effects
  • Regulatoraldosterone (excretion); insulin (intracellular shift)
  • Hypokalemiaflat T waves, U waves, muscle weakness, arrhythmia
  • Hyperkalemiapeaked T waves, wide QRS, cardiac arrest

Calcium (Ca²⁺)

  • Normal serum8.5-10.5 mg/dL (or ionized 1.1-1.3 mmol/L)
  • 99% in bone1% in blood; ionized fraction biologically active
  • RegulatorsPTH (raises Ca²⁺), calcitonin (lowers, minor), vitamin D (gut absorption)
  • Hypocalcemiatetany; Chvostek/Trousseau signs; QT prolongation
  • Hypercalcemiastones, bones, groans, psychiatric overtones

Phosphate (PO₄³-)

  • Inversely related to Ca²⁺PTH lowers phosphate
  • Vital in bone & ATPenergy currency

Acid-Base

Normal values

  • Arterial pH7.35-7.45 (tight!)
  • PaCO₂35-45 mmHg (respiratory component)
  • HCO₃-22-26 mEq/L (metabolic component)

Buffer systems (instant)

  • Bicarbonate (HCO₃-/H₂CO₃)main extracellular buffer
  • Phosphateintracellular and urinary
  • Protein (hemoglobin, plasma)absorbs or releases H+

Respiratory regulation (minutes)

  • Hyperventilationblows off CO₂; raises pH
  • Hypoventilationretains CO₂; lowers pH
  • Driven bymedullary chemoreceptors sensing CSF pH

Renal regulation (hours to days)

  • Excretes H+as titratable acid (phosphate buffer) and ammonium
  • Reabsorbs HCO₃-or generates new HCO₃- as needed
  • Slower but finallong-term compensator

Four primary disturbances

  • Metabolic acidosispH low, HCO₃- low · DKA, lactic acidosis, renal failure, diarrhea
  • Metabolic alkalosispH high, HCO₃- high · vomiting, diuretics, hyperaldosteronism
  • Respiratory acidosispH low, PaCO₂ high · COPD, narcotics, NM disease
  • Respiratory alkalosispH high, PaCO₂ low · anxiety, salicylates, high altitude

Anion gap

  • AG = Na+ - (Cl- + HCO₃-)normal ~12
  • High AG metabolic acidosisMUDPILES — methanol, uremia, DKA, propylene glycol, INH, lactic acidosis, ethylene glycol, salicylates
  • Normal AG (hyperchloremic)diarrhea, RTA
Dr. Sharilyn Rennie BIO 304 · Module 15 · Fluid, Electrolyte, Acid-Base